Article ID Journal Published Year Pages File Type
8418536 Journal of Immunological Methods 2013 4 Pages PDF
Abstract
► DNA vaccines of secreted and intracellular L. chagasi cysteine protease are compared ► N-terminal GM-CSFs were fused to the protease to enable secretion of the chimera ► Anti-protease antisera from immunized mice were evaluated by Western analysis ► Humoral responses were only detected when the protease was intentionally secreted ► The effect was attributed to secretion with no augmentation from GM-CSF activity
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