Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8418562 | Journal of Immunological Methods | 2012 | 8 Pages |
Abstract
The cryopreservation efficiency of the protein-free and fully chemically defined cryomedium was comparable to FBS-medium after storage for few weeks and several months. Directly after thawing, this medium yielded viabilities over 97% and recovery values over 84%. Also, the specific T-cell functionality was preserved. Additionally, short-term and six month cryopreservation gave comparable results. The fully chemically defined medium presented here will increase standardization and reproducibility of analysis in multicenter-studies or in retrospective evaluation.
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Authors
Julia C. Schulz, Anja Germann, Beatrice Kemp-Kamke, Angela Mazzotta, Hagen von Briesen, Heiko Zimmermann,