Article ID Journal Published Year Pages File Type
8418639 Journal of Immunological Methods 2012 10 Pages PDF
Abstract
► Quantitative glycan profiles of human IgG and its Fc-Fab portions are reported. ► Fab profile including the sialylated glycans is established for the first time. ► Fab profile contained highly sialylated and small amount of high-mannose glycans. ► Fc specific endoglycosidase S did not cleave the glycans with bisecting GlcNAc. ► Simple strategy for quantitative profiling of Fc and Fab glycans is described.
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