Article ID Journal Published Year Pages File Type
8418645 Journal of Immunological Methods 2012 5 Pages PDF
Abstract
► Brefeldin A (BFA) traps IL-4 within chlamydial-specific T cells. ► Monensin (MN) or BFA and MN prevents intracellular IL-4 accumulation. ► Simultaneous use of both inhibitors can be detrimental to ICS assays.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
Preview
Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis
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