Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8418651 | Journal of Immunological Methods | 2012 | 4 Pages |
Abstract
⺠Validation of reference genes is critical for accurate gene expression analysis. ⺠Ubc is the optimal reference gene for qPCR analysis of mouse B and T cells. ⺠Actb is not a suitable reference gene due to extensive variability in expression. ⺠Free software programs are available to rapidly validate potential reference genes.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Tina C. Albershardt, Brian M. Iritani, Alanna Ruddell,