Measurement of human latent transforming growth factor-Î²1 using a latency associated protein-reactive ELISA

Article ID	Journal	Published Year	Pages	File Type
8418717	Journal of Immunological Methods	2012	7 Pages	PDF

Abstract

âº Monoclonal antibodies were made against the LAP entity in human latent TGF-Î²1. âº A LAP ELISA specific for human latent TGF-Î²1, but not -Î²2 or -Î²3, was developed. âº The LAP ELISA quantified latent TGF-Î²1 in human samples without acid treatment. âº LAP ELISA analysis correlated with total TGF-Î²1 measurement by TGF-Î²1 ELISA. âº Bovine serum in human cell supernatants did not interfere with the LAP ELISA analysis.

Keywords

GFP TGF-β latent TGF-β binding protein LTBP LAP PBMC FBS mAb TGF-β1 PBS Monoclonal antibody recombinant human transforming growth factor-β ELISA fetal bovine serum peripheral blood mononuclear cells Cytokine Phosphate-buffered saline green fluorescent protein latency associated protein