Article ID Journal Published Year Pages File Type
8418730 Journal of Immunological Methods 2012 4 Pages PDF
Abstract
► A quick, cheap and effective way to purify lab-scale chicken antibodies from eggs. ► Using pectin, carrageenan and CaCl2 to delipidate and (NH4)2SO4 to precipitate IgY. ► We obtained approximately 60 mg of active antibody per egg yolk. ► The antibodies were approximately 80% pure and cost a fraction to isolate compared the commercial kits.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biotechnology
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