| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8418747 | Journal of Immunological Methods | 2012 | 9 Pages |
Abstract
Analysis of sera from mice immunized with different GBS vaccines revealed comparable sensitivity and specificity with the traditional killing OPA assay (kOPA), and a good correlation between the fluorescent signal of bacteria internalized by HL-60 phagocytes and killing. Remarkably, the pHrodo-based approach reduced the variability observed with other fOPA assays. The obtained data indicate the proposed fOPA as a reliable and useful tool for functional antibody assessment.
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Authors
Monica Fabbrini, Chiara Sammicheli, Immaculada Margarit, Domenico Maione, Guido Grandi, Marzia Monica Giuliani, Elena Mori, Sandra Nuti,