Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8418762 | Journal of Immunological Methods | 2012 | 7 Pages |
Abstract
⺠Anti-HIV-1 antibodies 3D6 and 4B3 were isotype switched from IgG to IgA. ⺠Recombinant CHO suspension cells stably expressed both IgAs serum-free. ⺠Different IgA expression qualified them as an interesting purification tool. ⺠We established and compared two IgA purification protocols. ⺠VHH ligands purified IgA irrespective of heavy/light chain at high purity and yield.
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Authors
David Reinhart, Robert Weik, Renate Kunert,