Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8418781 | Journal of Immunological Methods | 2012 | 6 Pages |
Abstract
⺠We sequence T cell receptors from a million T cells isolated from human blood samples. ⺠Spiking in T cell clones, we show the assay reliably detects 1 cell in 100,000. ⺠The assay is shown to precisely measure clone quantities from 10 to 100,000 T cells. ⺠We present a series of experiments to show that the assay is repeatable.
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Authors
Harlan Robins, Cindy Desmarais, Jessica Matthis, Robert Livingston, Jessica Andriesen, Helena Reijonen, Christopher Carlson, Gerold Nepom, Cassian Yee, Karen Cerosaletti,