Article ID Journal Published Year Pages File Type
8418850 Journal of Immunological Methods 2012 9 Pages PDF
Abstract
► Complement C3 deposition can mask array-bound IgG molecules from being detected. ► Modest dilution of blood delivers reliable data on IgG binding and C activation. ► Concentrations of bivalent cations need strict control for reproducible data. ► Properdin initiated AP activation is not suitable for normalizing C3 deposition. ► IgG capturing from serum can serve as reference protein for normalization.
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