Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8418850 | Journal of Immunological Methods | 2012 | 9 Pages |
Abstract
⺠Complement C3 deposition can mask array-bound IgG molecules from being detected. ⺠Modest dilution of blood delivers reliable data on IgG binding and C activation. ⺠Concentrations of bivalent cations need strict control for reproducible data. ⺠Properdin initiated AP activation is not suitable for normalizing C3 deposition. ⺠IgG capturing from serum can serve as reference protein for normalization.
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Authors
Krisztián Papp, Péter Végh, Renáta Hóbor, Anna Erdei, József Prechl,