Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8418853 | Journal of Immunological Methods | 2012 | 9 Pages |
Abstract
We here demonstrate that one hundred thousand T cells are sufficient to obtain a robust, highly reproducible measure of the global TCR Vβ repertoire diversity among twenty Vβ families in human peripheral blood. We also show that use of lower cell number results not in a dwindling of observed diversity but rather in non-reproducible patterns in replicate spectratypes. Finally, we report here a simple to use but sensitive method to quantify repertoire divergence in patient samples by comparison to a standard repertoire profile we generated from fifteen normal donors. We provide examples using this method to statistically evaluate the changes in the global TCR Vβ repertoire diversity that may take place during T subset immune reconstitution after hematopoietic stem cell transplantation or after immune modulating therapies.
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Authors
Sarfraz A. Memon, Claude Sportès, Francis A. Flomerfelt, Ronald E. Gress, Frances T. Hakim,