Advanced flowcytometric analysis of regulatory T cells: CD127 downregulation early post stem cell transplantation and altered Treg/CD3+CD4+-ratio in severe GvHD or relapse

Article ID	Journal	Published Year	Pages	File Type
8419100	Journal of Immunological Methods	2011	9 Pages	PDF

Abstract

âº This study showed a decreased CD127 expression on T cells in patients post-SCT. âº Higher absolute Tregs in patients with GvHDÂ â¤Â grade I compared to those with II-IV. âº The Treg percentage was higher in patients with GvHD grade II-IV or disease relapse. âº A small advantage was observed applying additional CD127 in Treg determination. âº Advanced flowcytometry for naïve, effector- and central memory Treg detection.

Keywords

MMF PC5 AML GvHD mismatched unrelated donor DLI PC7 PBSC MFD MTX BMT APC int GMP ECD mAb FITC MFI natural killer allophycocyanin Monoclonal antibody Matched unrelated donor CSA High Donor lymphocyte infusion Good Manufacturing Practice intermediate Peripheral blood Peripheral blood stem cells cyclosporin A fluorescein isothiocyanate acute myeloid leukemia Acute lymphoblastic leukemia mycophenolate mofetil Methotrexate bone marrow mean fluorescence intensity ALL body weight Graft versus host disease Bone marrow transplantation low Mud

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Advanced flowcytometric analysis of regulatory T cells: CD127 downregulation early post stem cell transplantation and altered Treg/CD3+CD4+-ratio in severe GvHD or relapse

Authors

Melanie Bremm, Sabine Huenecke, Thomas Lehrnbecher, Eva Ponstingl, Regine Mueller, Annekathrin Heinze, Gesine Bug, Andrea Quaiser, Michael Kapinsky, Claudia Brehm, Peter Bader, Gisbert Schneider, Thomas Klingebiel, Ulrike Koehl,