| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8419163 | Journal of Immunological Methods | 2011 | 9 Pages |
Abstract
⺠CpG ODN stimulation expands the mouse spleen B cell in short period. ⺠Antibody producing cells are stimulated preferentially among B cells. ⺠Stimulated cells are electrofused with myeloma cells with the higher efficiency. ⺠Cell activation by CpG ODN increases the number of positive hybridoma. ⺠A functional antibody against the luciferase was established with this method.
Keywords
PBSCpG oligodeoxynucleotideCpG ODNIL-12GM-CSFIL-2IFN-γIL-10PWMPBMCFBSLPSElectrofusioninterferon-γinterleukin-12Interleukin-10Interleukin-2Blastogenesistumor necrosis factor-αfetal bovine serumTNF-αPhosphate buffered salineluciferaselipopolysaccharidepokeweed mitogengranulocyte macrophage colony-stimulating factorHybridomahypoxanthineMonoclonal antibodiespolyethyleneglycolPEGHATToll-like receptor 9
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Authors
Mieko Kato, Eriko Sasamori, Tomoki Chiba, Yoshiro Hanyu,