| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8419184 | Journal of Immunological Methods | 2011 | 7 Pages |
Abstract
⺠Immature DCs are used to investigate the immunomodulatory properties of probiotics. ⺠We standardized a method to efficiently generate iDCs. ⺠Cryopreservation would simplify and standardize functional studies on DCs. ⺠Cryopreservation affects dendritic cell cytokine responses depending on stimulus. ⺠Cryopreserved cells produce up to 250 fold less IL-12 in response to bacteria.
Keywords
CFULPSIMDMLTAGM-CSFIDCPBMCsIFN-γIscove's modified Dulbecco's mediumlipoteichoic acidFreezinginterferon-gammainterleukinMaturationImmunomodulationtumor necrosis factor-alphaCryopreservationDendritic cellImmature dendritic cellperipheral blood mononuclear cellsDendritic cellsTNF-αLamina proprialipopolysaccharideMAMPSgranulocyte macrophage-colony stimulating factorcolony forming unitsProbiotics
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Authors
Marjolein Meijerink, Dulantha Ulluwishewa, Rachel C. Anderson, Jerry M. Wells,