Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8419220 | Journal of Immunological Methods | 2011 | 7 Pages |
Abstract
⺠An efficient isolation procedure for nuclei of primary immune cells is described. ⺠Living cells are applied on an iodixanol-based density gradient that contains a lysis layer. ⺠Mild cell lysis and washing of the nuclei is performed during a single low speed centrifugation step in only 10 min. ⺠Highly pure nuclei can be obtained from only 1 Ã 106 primary cells such as monocytes.
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Authors
Marko Poglitsch, Karl Katholnig, Marcus D. Säemann, Thomas Weichhart,