| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8419237 | Journal of Immunological Methods | 2011 | 8 Pages |
Abstract
⺠We developed a standardized ELISA to quantify soluble FcεRI in human serum. ⺠Specificity and precision tests met acceptance criteria for immunoassay validation. ⺠We further validated our ELISA in an established pediatric patient cohort. ⺠Serum soluble FcεRI correlates with IgE in patients with elevated serum IgE. ⺠This ELISA can now be used to study the role of soluble FcεRI in health and disease.
Keywords
TMBHRPIgEkDaNIPBCAIgGLLOQFBSmAbPBSbicinchoninic acid assayAllergyMonoclonal antibodystandard deviationSerum biomarkerImmunoprecipitationELISAEnzyme-linked immunosorbent assaylower limit of quantificationfetal bovine serumCoefficient of VariationPhosphate-buffered salineHorseradish peroxidaseoptical densitykilodaltonsImmunoglobulin gammaFc receptor
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Authors
Willem Lexmond, John van der Mee, Floortje Ruiter, Barbara Platzer, Georg Stary, Elizabeth H. Yen, Eleonora Dehlink, Samuel Nurko, Edda Fiebiger,