Protein overexpression following lentiviral infection of primary mature neutrophils is due to pseudotransduction

► In-depth comparison of lentiviral transduction phenomena between primary neutrophils and Jurkat T cells. ► Lentiviral infection of mature neutrophils results in protein overexpression due to pseudotransduction. ► Protein transfer and not gene transfer is responsible for neutrophil pseudotransduction at early time-points of infection. ► Protein pseudotransduced into mature neutrophils is non-functional. ► Lentiviral transduction cannot be used to manipulate mature neutrophils.