| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8419316 | Journal of Immunological Methods | 2011 | 13 Pages |
Abstract
ⶠWe have re-cloned the ZnT8-R cDNA into a high efficiency in vitro transcription-translation plasmid. ⶠWe have subjected the ZnT8-R cDNA to site-directed mutagenesis to generate the ZnT8-W and ZnT8-Q variants. ⶠWe have tested a novel ZnT8-TripleA assay to screen for circulating ZnT8A against any of the three variants. ⶠThe ZnT8-TripleA assay showed a low false positive rate and a negligible false negative rate. ⶠThis assay represents a highly reproducible practical alternative to individual RBA in newly diagnosed diabetes children.
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Authors
Fariba Vaziri-Sani, Ahmed J. Delli, Helena Elding-Larsson, Bengt Lindblad, Annelie Carlsson, Gun Forsander, Sten A. Ivarsson, Johnny Ludvigsson, Claude Marcus, Ã
ke Lernmark,