| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8419325 | Journal of Immunological Methods | 2011 | 10 Pages |
Abstract
⺠We describe a new procedure, Nano-iPCR, for detection of cytokines using functionalized gold nanoparticle-based immuno-PCR. ⺠In this system, DNA template and target antigen-specific antibody are conjugated through gold nanoparticles. ⺠Nano-iPCR is superior in sensitivity and detection range than ELISA and is easier to perform than iPCR and ELISA. ⺠Nano-iPCR may be preferable used for rapid detection of low concentrations of cytokines in complex biological fluids.
Keywords
HRPTPBSPBSiPCRDMEMFBSBMMCSCFBSADulbecco's modified Eagle Mediumo-PhenylenediaminePBS containing 0.05% Tween 20Real-time PCRopdbovine serum albuminimmuno-PCRinterleukinRecombinantELISAEnzyme-linked immunosorbent assayfetal bovine serumbone marrow-derived mast cellStem Cell FactorMast cellphosphate buffer solutionreverse-transcriptionGold nanoparticlepolymerase chain reactionPCRHorseradish peroxidasequantification cycle
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Authors
Lucie PotůÄková, Filip Franko, Monika Bambousková, Petr Dráber,