Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8419326 | Journal of Immunological Methods | 2011 | 13 Pages |
Abstract
⺠Monoclonal anti-Gb3, clone 38-13, is superior to other monoclonal anti-Gb3 for detection of cell-surface Gb3. ⺠VT1B conjugated to a fluorochrome provides optimal detection of cell-surface Gb3. ⺠Crossreactivity of VT1B with Gb4 can occur when Gb4 levels are very high.
Keywords
Gb3Alexa488mAbCD77PBMCFITCMFIFSCRhamnose-binding lectinSSCTLCGSLRBLglycosphingolipidMonoclonal antibodyEBVinterferonIFNHUSperipheral blood mononuclear cellsHemolytic uremic syndromephycoerythrinfluorescein isothiocyanateFlow cytometrymajor histocompatibility complexMHCmean fluorescence intensityverotoxinEpstein–Barr virushuman immunodeficiency virusHIVforward scatterside scatterthin layer chromatographyglobotriaosylceramide
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Authors
Minji Kim, Beth Binnington, Darinka Sakac, Kimberly R. Fernandes, Sheryl P. Shi, Clifford A. Lingwood, Donald R. Branch,