| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8419399 | Journal of Immunological Methods | 2011 | 7 Pages |
Abstract
⺠A novel method for the transformation of macrophages with DNA was developed. ⺠IgG-opsonized lipid-based microbubbles bearing plasmid DNA were synthesized. ⺠Phagocytosed microbubbles facilitate sonoporation of the phagosome by ultrasound. ⺠Modest transfection efficiency but well tolerated by cells. ⺠Method can be used in vitro and in vivo.
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Authors
Jason C.M. Lemmon, Ryan J. McFarland, Joanna M. Rybicka, Dale R. Balce, Kyle R. McKeown, Regina M. Krohn, Terry O. Matsunaga, Robin M. Yates,