Flow cytometric mast cell-mediated cytotoxicity assay: A three-color flow cytometric approach using monoclonal antibody staining with annexin V/propidium iodide co-labeling to assess human mast cell-mediated cytotoxicity by fluorosphere-adjusted counts

This technique is reliable, is more sensitive and has some advantages no less than the evaluation of early apoptosis and exact total cell loss. Our findings suggest MC's anti-tumor role and these in vitro studies help enlighten MC's interactions with tumor cells.