| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8419965 | Journal of Immunological Methods | 2009 | 6 Pages |
Abstract
To detect BPA residue in the environment quickly by immune method, preparation of high quality antibody against BPA was the most critical step of project. Based on Mannich-type reaction and EDC method, we produced BPA-cBSA and BHPVA-BSA against BPA and the conjugates were characterized by UV-Vis and FT-IR spectroscopy. Compared with BSA, cBSA as carrier protein could improve the efficiency of coupling and significantly enhance the immune response against BPA. The mole coupling ratio of BPA-cBSA (8:1) was greater than that of BHPVA-BSA (5:1). We also observed that the similar sensitivity of antisera against BPA between the BPA-cBSA group and BHPVA-BSA group.
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Authors
Yi Feng, Youxiang Zhou, Qiang Zou, Jixing Wang, Fusheng Chen, Zhixian Gao,