Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8420246 | Journal of Immunological Methods | 2008 | 6 Pages |
Abstract
Interest in plasma cells has increased greatly in the past decade. While several studies have examined the longevity and transcriptional control of antibody secreting cells in vivo, few studies have examined freshly isolated plasma cells ex vivo. Studies of primary plasma cells have been limited primarily due to the difficulty of isolating the large numbers of plasma cells necessary for experiments. In this protocol, plasma cells are purified from murine bone marrow in approximately 5Â hours, following a two-step isolation method utilizing column enrichment and FACS-sorting based on CD138 (Syndecan-1) surface expression.
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Authors
Heather A. Minges Wols, Pamela L. Witte,