Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8426002 | Synthetic and Systems Biotechnology | 2017 | 20 Pages |
Abstract
Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities. Polyketides encompass one of the largest classes of chemically diverse natural products. With thousands of known polyketides, modular polyketide synthases (PKSs) share a particularly attractive biosynthetic logic for generating chemical diversity. The engineering of modular PKSs could open access to the deliberate production of both existing and novel compounds. In this review, we discuss PKS engineering efforts applied at both the protein and cellular level for the generation of a diverse range of chemical structures, and we examine future applications of PKSs in the production of medicines, fuels and other industrially relevant chemicals.
Keywords
propionyl-CoA carboxylase6-deoxyerythronolide B synthaseDEBsTKLN-acetylcysteamineLTTRLysR-Type Transcriptional RegulatorPolyketideSARPsNAcReductase domainPKSACPFASPDBAcyltransferasePCCThioesteraseDehydratasePolyketide synthaseSynthetic biologyNatural productsMetabolic engineeringacyl carrier proteinketosynthaseKetoreductasecol
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Authors
Jesus F. Barajas, Jacquelyn M. Blake-Hedges, Constance B. Bailey, Samuel Curran, Jay. D. Keasling,