Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8430041 | Biology of Blood and Marrow Transplantation | 2018 | 10 Pages |
Abstract
- Quantification of mixed chimerism is increasingly important in HSCT and cellular therapies.
- Current methods lack sensitivity in the microchimerism range (<1%) and have drawbacks such as technical complexity and slow turnaround time.
- We have developed a highly sensitive droplet digital PCR assay for accurate quantification of chimerism throughout the clinical range including microchimerism to a limit of detection of .008% and limit of quantification of .023%.
- We have successfully utilized the assay in clinical samples of patients undergoing HSCT, in microtransplantation and in HSCT patients treated with third-party virus-specific T cells.
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Authors
David Kliman, Gloria Castellano-Gonzalez, Barbara Withers, Janine Street, Elizabeth Tegg, Oksana Mirochnik, Joey Lai, Leighton Clancy, David Gottlieb, Emily Blyth,