Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8438762 | EBioMedicine | 2017 | 11 Pages |
Abstract
Obesity and its related metabolic disorders are closely correlated with gut dysbiosis. Montmorillonite is a common medicine used to treat diarrhea. We have previously found that dietary lipid adsorbent-montmorillonite (DLA-M) has an unexpected role in preventing obesity. The aim of this study was to further investigate whether DLA-M regulates intestinal absorption and gut microbiota to prevent obesity-related metabolic disorders. Here, we show that DLA-M absorbs free fatty acids (FFA) and endotoxins in vitro and in vivo. Moreover, the combination of fluorescent tracer technique and polarized light microscopy showed that DLA-M crystals immobilized BODIPY® FL C16 and FITC-LPS, respectively, in the digestive tract in situ. HFD-fed mice treated with DLA-M showed mild changes in the composition of the gut microbiota, particularly increases in short-chain fatty acids (SCFA)-producing Blautia bacteria and decreases in endotoxin-producing Desulfovibrio bacteria, these changes were positively correlated with obesity and inflammation. Our results indicated that DLA-M immobilizes FFA and endotoxins in the digestive tract via the adsorption-excretion axis and DLA-M may potentially be used as a prebiotic to prevent intestinal dysbiosis and obesity-associated metabolic disorders in obese individuals.
Keywords
SCFAHFDQUICKIFFANEFARDAPCoAHOMA-IRNCDOGTTIBSNAFLDIGTTVFAT2DLPSinsulin tolerance testOral glucose tolerance testMetabolic disordersOleic acidFree fatty acidvolatile fatty acidshort-chain fatty acidFree fatty acidsnon-esterified fatty acidsEndotoxinsITTWhite adipose tissueepididymal white adipose tissuemesenteric white adipose tissueOUTNonalcoholic fatty liver diseaseredundancy analysisPrincipal Coordinates AnalysistriglycerideType 2 diabetesHigh fat dietnormal chow dietirritable bowel syndromelipopolysaccharideObesityMontmorilloniteGut microbiotaoperational taxonomic unitPalmitic acidWATtotal cholesterol
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cancer Research
Authors
Pengfei Xu, Fan Hong, Jialin Wang, Yusheng Cong, Shu Dai, Sheng Wang, Jing Wang, Xi Jin, Fang Wang, Jin Liu, Yonggong Zhai,