Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8455614 | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | 2018 | 15 Pages |
Abstract
The present study was aimed to explore the protective role of Aloe vera gel extract against hepatic and renal damage caused by X-ray exposure to mice. Male balb/c mice were divided into four groups: control, Aloe vera gel extract [AV] (50â¯mg/ kg b.w on alternate days for 30 days), X-ray (2â¯Gy) and AVâ¯+â¯X-ray. X-ray irradiation enhanced the serum levels of liver function indices and chromosomal abnormalities in liver. Kidney function markers were found to be deranged and were accompanied by reduced glomerular filtration rate indicating renal dysfunction. Irradiation caused histopathological and biochemical alterations in both tissues which was associated with enhanced reactive oxygen species (ROS), lipid peroxidation (LPO) levels, lactate dehydrogenase (LDH) activity and enhanced apoptosis as revealed by TUNEL assay and DNA fragmentation. The administration of Aloe vera gel extract to X-ray exposed animals significantly improved their hepatic and renal function parameters which were associated with a reduction in ROS/LPO levels, LDH activity and chromosomal abnormalities as compared to their irradiated counterparts. In vitro assays revealed effective radical scavenging ability of Aloe vera gel extract, which may be linked to its potential in exhibiting antioxidant effects in in vivo conditions. This data suggested that Aloe vera may serve to boost the antioxidant system, thus providing protection against hepatic and renal damage caused by X-ray.
Keywords
SGOTNBTGSSGDTNBCDNBLPODAPGSTGSHEtBrNADPHGFRCATSGPTABTSDAB99mTc-DTPAAlbumin globulin ratioBHTDPPHtris-acetate-EDTAterminal deoxynucleotidyl transferase mediated dUTP nick end labeling1-chloro-2, 4-dinitrobenzene5,5′-dithiobis-(2-nitrobenzoic acid)A/GGSH-PxH&EROST-BILHydrogen peroxideAloe veraethidium bromideEDTAEthylene diamine tetra acetic acidDirect bilirubinnitro blue tetrazoliumChromosomal analysisSerum glutamic oxaloacetic transaminaseserum glutamic pyruvic transaminaseTAETUNELGrayBUNdiaminobenzidineSODSuperoxide dismutaseAntioxidant defense systemlactate dehydrogenaseLDHdose area productNADHGlomerular filtration rateblood urea nitrogennicotinamide adenine dinucleotide reducednicotinamide adenine dinucleotide phosphate reducedhaematoxylin and eosinH2O2butylated hydroxytolueneinternational unitsLipid peroxidationCatalaseCreatcreatinineglutathione-S-transferaseglutathione oxidizedglutathione reductaseglutathione peroxidaseGlutathione reducedReactive oxygen species
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Authors
Shashi Bala, Neha Arora Chugh, Subhash Chand Bansal, Mohan Lal Garg, Ashwani Koul,