Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8455626 | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | 2018 | 7 Pages |
Abstract
The psychrophilic microalga, Chlamydomonas sp. ICE-L, isolated from floating ice in the Antarctic, one of the most highly UV exposed ecosystems on Earth, displays an efficient DNA photorepair capacity. Here, the first known (6-4) photolyase gene (6-4CiPhr) from C. sp. ICE-L was identified. The 6-4CiPhr encoded 559-amino acid polypeptide with a pI of 8.86, and had a predicted Mw of 64.2â¯kDa. Real-time PCR was carried out to investigate the response of 6-4CiPhr to UVB exposure. The transcription of 6-4CiPhr was up-regulated continuously within 6â¯h, achieving a maximum of 62.7-fold at 6â¯h. Expressing 6-4CiPhr in a photolyase-deficient Escherichia coli strain improved survival rate of the strain. In vitro activity assays of purified protein demonstrated that 6-4CiPhr was a photolyase with 6-4PP repair activity. These findings improve understanding of photoreactivation mechanisms of (6-4) photolyase.
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Authors
Meiling An, Zhou Zheng, Changfeng Qu, Xixi Wang, Hao Chen, Chongli Shi, Jinlai Miao,