Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8455695 | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | 2015 | 8 Pages |
Abstract
The opportunistic human fungal pathogen Candida albicans is a natural diploid that does not have a complete sexual cycle. The ability to switch between diverse cellular forms is important to its virulence. Here, we describe the characterization of the C. albicans DBF4 gene, a Saccharomyces cerevisiae homolog that encodes a regulatory subunit of Cdc7 kinase that is known to initiate DNA replication. We made a C. albicans strain, with one DBF4 allele deleted by the mini-Ura-blaster and the other controlled by a repressible promoter. We also found a third CaDBF4 copy that was later verified to be inducibly duplicated by targeted recombination with the min-Ura-blaster. Surprisingly, the strain deleted with the third CaDBF4 copy exhibited hyphal growth under repressed conditions. We conclude that the CaDBF4 gene is prone to being duplicated by the mini-Ura-blaster and that it suppresses hyphal growth in C. albicans.
Keywords
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Authors
Ting Chien, Tzu-Ling Tseng, Jiun-Yuan Wang, Yi-Ting Shen, Ting-Hui Lin, Jia-Ching Shieh,