Mechanisms involved in alternariol-induced cell cycle arrest

Article ID	Journal	Published Year	Pages	File Type
8455881	Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis	2012	11 Pages	PDF

Abstract

âº The mycotoxin alternariol induce DNA strand breaks as well as oxidative DNA damage. âº A DNA damage response was activated, involving H2AX, Chk-1, Chk-2 and p53. âº Changes in p21, MDM2, Cyclin B1 and miRNA may explain the induced cell cycle arrest. âº AOH induced ROS was not linked to the cell cycle arrest. âº ROS-induced Sestrin 2 might contribute to oxidant defense.

Keywords

FPG HRP qRT-PCR AOH AME Alternariol monomethyl ether NMPA DSBs TBST JC-1 PFA BRCA 8-oxoGua PCNA LMPA PFT-α DNA double stranded breaks pifithrin-α TBS-tween NAC TBS p53 ASC ATR PBS 2′,7′-dichlorodihydrofluorescein diacetate 8-oxo-7,8-dihydroguanine ataxia telangiectasia mutated BSA Mdm2 N-acetyl-cysteine ROS SSBs Ascorbic acid DNA damage bovine serum albumin alternariol Proliferating Cell Nuclear Antigen Tenuazonic acid analysis of variance ANOVA Tris-buffered saline ATM murine double minute 2 dihydroethidium Breast cancer Immunotoxicity formamidopyrimidine DNA glycosylase Phosphate buffered saline Mycotoxins MicroRNA MiRNA normal melting point agarose low melting point agarose DHE quantitative real time polymerase chain reaction paraformaldehyde horse radish peroxidase Propidium iodide TEA Reactive oxidant species