Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8456244 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2018 | 6 Pages |
Abstract
This study aimed at analyzing the frequency of micronuclei (MN) in exfoliated cells as well as the levels of Pb-210 in urine samples to evaluate the association between the smoking habit and toxic stress of transitional epithelial cells. The frequency of MN was scored from Giemsa-stained slides while exchange resin and beta counting techniques were employed to measure the concentrations of this radioisotope. Urine samples of smokers had levels of Pb-210 up to 158.65â¯mBqâ¯Lâ1. For nonsmokers, the median was below the detection limit (45â¯mBqâ¯Lâ1). The analyses of mononucleated cells showed a significant increase of the frequency of MN in smokers when compared to nonsmokers. Statistical tests showed a tight relation between the cigarette consumption and the increase of the frequency of MN, rather than with the levels of Pb-210 present in smoke particles. The results indicate the usefulness of the methodology for the evaluation of human health risks related to chronic contamination with Pb-210.
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Authors
Carlos Eduardo de Oliveira Costa Júnior, Leone Maltz Borges Silva, Thiago de Salazar e Fernandes, Alex Souza Moraes, Ademir Amaral, Edvane Borges Edvane Borges,