Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8456253 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2016 | 38 Pages |
Abstract
A range of fibrous materials, including several types of asbestos and carbon fibres with nano scale diameters that had reported positive genotoxicity data (predominantly clastogenicity), were tested in the in vitro micronucleus test (OECD 487) in GLP-compliant studies in Chinese Hamster Ovary cells. Out of eight materials tested, only one (crocidolite, an asbestos fibre) gave a positive response either in the presence or absence of metabolic activation (S9) and at short (3 h) or extended (24 h) exposure times (p â¤Â 0.001). Our data suggest that the commonly used tests for clastogenicity in mammalian cells require extensive modification before fibrous materials are detected as positive, raising questions about the validity of these tests for detecting clastogenic and aneugenic fibrous materials.
Keywords
glutathione S-transferase mu 1GLPUICCMWCNTSWCNTG6PHprtSCEOECDMLAGSTM1CNFMnBnChlDMSOROSMouse lymphoma assayIn vitro micronucleus testChromosome aberration testUnion for International Cancer ControlEDTAEthylenediaminetetraacetic acidFibreSHETemSister chromatid exchangeGood Laboratory PracticeSyrian hamster embryoSensitivityDimethylsulfoxideChinese hamster lungthe Organisation for Economic Co-operation and DevelopmentHuman cellsGenotoxicityreplication indexNano materialMicronucleusTransmission electron microscopysingle walled carbon nanotubeglucose-6-phosphateReactive oxygen species
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cancer Research
Authors
Paul Fowler, Andrew Homan, Derek Atkins, James Whitwell, Melvyn Lloyd, Roberta Bradford,