| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8456303 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2015 | 22 Pages |
Abstract
Titanium dioxide nanoparticles (TiO2 NPs) are widely used for their whiteness and opacity. We investigated the hematological effects and genotoxicity of anatase TiO2 NPs following sub-chronic oral gavage treatment. TiO2-NPs were characterized by X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). Wistar rats were treated with anatase TiO2 NPs by intragastric administration for 60 days. Hematological analysis showed a significant decrease in RBC and HCT and a significant increase in MCV, PLT, MPV and WBC at higher doses. Furthermore, abnormally shaped red cells, sometimes containing micronuclei, and hyper-segmented neutrophil nuclei were observed with TiO2 NPs treatment. The micronucleus test revealed damage to chromosomes in rat bone marrow at 100 and 200Â mg/kg bw; the comet assay showed significant DNA damage at the same doses.
Keywords
RBCMN-PCEMCVMPVMCHCPLTMCHHCTWBCEDXRed blood cellsmicronucleated polychromatic erythrocytesTiO2 NPsDNA damageTemANOVAMean corpuscular volumetail momentGenotoxicityHematopoietic systemtail lengthX-ray photoelectron spectroscopyXPSmean platelet volumemean corpuscular hemoglobinRatMean corpuscular hemoglobin concentrationTransmission electron microscopyTitanium dioxide nanoparticleshematocritHemoglobinHgbbody weightX-ray diffractionXRDPlateletsAnemiawhite blood cellsone way analysis of variance
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Authors
Intissar Grissa, Jaber Elghoul, Lobna Ezzi, Sana Chakroun, Emna Kerkeni, Mohsen Hassine, Lassaad El Mir, Meriem Mehdi, Hassen Ben Cheikh, Zohra Haouas,