Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8456386 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2015 | 7 Pages |
Abstract
In vivo comet assay results are more reliable if they were obtained in laboratories that have demonstrated proficiency. This includes demonstration of adequate response to vehicle controls and an adequate response to a positive control for each tissue being examined. There was a general agreement that freezing of samples is an option but more data are needed in order to establish generally accepted protocols. With regard to tissue toxicity, the working group concluded that cytotoxicity could be a confounder of comet results. It is recommended to look at multiple parameters such as histopathological observations, organ-specific clinical chemistry as well as indicators of tissue inflammation to decide whether compound-specific toxicity might influence the result. The expert working group concluded that the alkaline in vivo comet assay is a mature test for the evaluation of genotoxicity and can be recommended to regulatory agencies for use.
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Authors
Günter Speit, Hajime Kojima, Brian Burlinson, Andrew R. Collins, Peter Kasper, Ulla Plappert-Helbig, Yoshifumi Uno, Marie Vasquez, Carol Beevers, Marlies De Boeck, Patricia A. Escobar, Sachiko Kitamoto, Kamala Pant, Stefan Pfuhler, Jin Tanaka,