| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8456472 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2014 | 6 Pages |
Abstract
Like 8-oxoGua, 5-hydroxymethyluracil (5-hmUra) is another oxidatively modified DNA base (the derivative is formed by thymine oxidation). Recent experimental evidence supports the notion that 5-hmUra plays an important role in active DNA demethylation. This involves overexpression of activation-induced cytidine deaminase (AID) and ten-eleven translocation 1 (TET1) protein (the key proteins involved in active demethylation), which leads to global accumulation of 5-hmUra. Our preliminary data demonstrate a significant increase of the 5-hmUra levels in pig brain extract when compared with liver extract. The lack of 5-hmUra in Escherichia coli DNA also speaks for a role of this modification in the active demethylation process. It is concluded that 8-oxodG and 5-hmUra in DNA may be considered as epigenetic marks.
Keywords
8-oxoGua5-methyl-2′-deoxycytidineActive DNA demethylation8-Oxoguanine glycosylaseActivation-induced cytidine deaminaseOGG1TDG8-oxodG5-Hydroxymethyl-2′-deoxyuridine5-Hydroxymethyluracil5-Methylcytosine5-hydroxymethylcytosine8-oxo-7,8-dihydro-2′-deoxyguanosine8-oxoguanineGene expressionEpigenetic changesAID
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Authors
Ewelina Zarakowska, Daniel Gackowski, Marek Foksinski, Ryszard Olinski,