Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8456531 | Mutation Research/Genetic Toxicology and Environmental Mutagenesis | 2013 | 7 Pages |
Abstract
To analyze translesion DNA synthesis (TLS) across lesions derived from the air pollutant 3-nitrobenzanthrone in Escherichia coli, we constructed site-specifically modified plasmids containing single molecule adducts derived from 3-nitrobenzanthrone. For this experiment, we adopted a modified version of the method developed by Fuchs et al. [29]. Each plasmid contained one of the following lesions in its LacZâ² gene: N-(2â²-deoxyguanosin-8-yl)-3-aminobenzanthrone (dG-C8-N-ABA); 2-(2â²-deoxyguanosin-N2-yl)-3-aminobenzanthrone (dG-N2-C2-ABA); 2-(2â²-deoxyguanosin-8-yl)-3-aminobenzanthrone (dG-C8-C2-ABA); 2-(2â²-deoxyadenosin-N6-yl)-3-aminobenzanthrone (dA-N6-C2-ABA); N-(2â²-deoxyguanosin-8-yl)-3-acetylaminobenzanthrone (dG-C8-N-AcABA); or 2-(2â²-deoxyguanosin-8-yl)-3-acetylaminobenzanthrone (dG-C8-C2-AcABA). All of the adducts inhibited DNA synthesis by replicative DNA polymerases in E. coli; however, the extent of the inhibition varied among the adducts. All five dG-adducts strongly blocked replication by replicative DNA polymerases; however, the dA-adduct only weakly blocked DNA replication. The induction of the SOS response increased the frequency of TLS, which was higher for the dG-C8-C2-ABA, dG-C8-N-AcABA and dG-C8-C2-AcABA adducts than for the other adducts. In our previous study, dG-C8-N-ABA blocked DNA replication more strongly and induced mutations more frequently than dG-N2-C2-ABA in human cells. In contrast, in E. coli the frequency of TLS over dG-N2-C2-ABA was markedly reduced, even under the SOS+ conditions, and dG-N2-C2-ABA induced G to T mutations. All of the other adducts were bypassed in a less mutagenic manner. In addition, using E. coli strains that lacked particular DNA polymerases we found that DNA polymerase V was responsible for TLS over dG-C8-N-AcABA and dG-C8-C2-AcABA adducts.
Keywords
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Authors
Masanobu Kawanishi, Takaharu Kanno, Hiroshi Nishida, Tekeji Takamura-Enya, Takashi Yagi,