Acrylamide-induced alterations in the cocaine- and amphetamine-regulated peptide transcript (CART)-like immunoreactivity within the enteric nervous system of the porcine small intestines

The main goal of the present study was to determine the influence of low and high doses of acrylamide on CART-like immunoreactivity within the ENS of the porcine small intestines. Investigation was performed on 15 sexually immature female pigs, divided into three groups: control group, where empty gelatin capsules were administered, and two experimental groups, where capsules containing low or high doses of acrylamide were given. After 28 days of acrylamide exposure all animals were euthanized with a gradual overdose of anaesthetics. Immediately after euthanasia fragments of the duodenum, jejunum and ileum were collected and fixed. Then, 14 μm-thick cryostat sections were processed for routine double-labelling immunofluorescence using primary antisera directed towards a pan neuronal marker - protein gene-product 9.5 (PGP 9.5) and cocaine- and amphetamine-regulated peptide transcript (CART). During the present investigation, CART-LI cell bodies were detected in all types of enteric plexuses of duodenum, jejunum and ileum. Acrylamide intoxication resulted in a significant increase in expression of CART in the intramural neurons of the porcine small intestines, especially in myenteric plexuses. It may suggest participation of CART in neuronal protection and recovery processes within the gut. Moreover, results of the present study suggest that even low doses of acrylamide cause a significant response of ENS neurons.