Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8461815 | Annals of Anatomy - Anatomischer Anzeiger | 2012 | 6 Pages |
Abstract
The aim of this study was to investigate the effects of BONITmatrix® and OSSA NOVA on the expression of growth factors and osteogenic differentiation. For this purpose, the mRNA expression of VEGF, IGF1, IGF2, collagen-1, collagen-2 and MMP8 was analysed in surgically created defects on the crania of adult male rats. Cranial samples were collected after implantation of BONITmatrix® or OSSA NOVA scaffolds for 4 weeks and determinations of gene expression were performed by quantitative RT-PCR. Real-time RT-PCR analyses showed a significantly higher expression of IGF1 in both groups treated with BONITmatrix® and OSSA NOVA compared to untreated controls, whereas type I collagen mRNA expression only increased in BONITmatrix® treated rats compared to controls. No changes in transcript expression of IGF2, VEGF, collagen-2 and MMP8 were detectable between the analysed groups. In conclusion, BONITmatrix® and OSSA NOVA stimulate the expression of growth factor IGF1, but only the granular dosage form is able to stimulate osteoblast differentiation.
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Authors
Tomasz Gredes, Friedhelm Heinemann, Marzena Dominiak, Heike Mack, Tomasz Gedrange, Alexander Spassov, Thomas Klinke, Christiane Kunert-Keil,