Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8462369 | Annals of Anatomy - Anatomischer Anzeiger | 2009 | 19 Pages |
Abstract
Cryo-electron tomography of vitrified samples at cryogenic temperatures combines excellent structural preservation with direct high-resolution imaging. The use of cryo-preparation and imaging techniques eliminates artifacts induced by plastic embedding and staining of the samples is circumvented. This review describes the technique of cryo-electron tomography, its basic principles, cryo-specimen preparation, tomographic data acquisition and image processing. A number of illustrative examples ranging from whole cells, cytoskeletal filaments, viruses and organelles are presented along with a comprehensive list of research articles employing cryo-electron tomography as the key ultrastuctural technique.
Keywords
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Authors
Roman I. Koning, Abraham J. Koster,