Automated microscopic quantification of adipogenic differentiation of human gland stem cells

We developed a method of determining differentiation efficiencies covering the range from 10% down to 100 ppm with the same image processing and an identical set of parameters, matching the results of the adipogenesis assay. Our approach is based on a statistically significant number of cells and shows high sensitivity taking into account the heterogeneous differentiation pattern of adipogenesis in GSC and other stem cells.