Influence of cell culture media conditions on the osteogenic differentiation of cord blood-derived mesenchymal stem cells

We found that MSCGM, 10% SingleQuot had a supportive effect on the osteogenic differentiation of UCB-MSCs. In case of treatment with 10−8 M dexamethasone, mineralization occurred in combination with BMP-2 exclusively, while a concentration of 10−7 M dexamethasone led to a high amount of mineralized ECM and the expression of collagen-1 independent of BMP-2 addition. According to this data dexamethasone is the leading osteoinductive factor, but BMP-2 seems to have supportive properties in UCB-MSCs. In conclusion, MSCGM supplemented with 10% SingleQuot and 10−7 M dexamethasone was the condition identified to be best for inducing the osteogenic differentiation of UCB-MSCs.