Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8463660 | Cellular Immunology | 2017 | 9 Pages |
Abstract
Langerhans cells (LCs) from melanoma patients sentinel lymph nodes (SLN) are poor T cell activators mostly due to an immature immunophenotype. However Antigen Presenting Machinery (APM) role is unknown. We investigated HLA-class I APM components (Delta, LMP-7/10, TAP-1, Calnexin, Tapasin, β2-microglobulin and HLA-A,B,C) in LCs from healthy donors skin and melanoma patients SLN. APM component levels were low in immature epidermal LCs and significantly increased after maturation (p < 0.05); their levels were significantly high in SLN LCs (p < 0.01). APM component expression correlated with melanoma Breslow's thickness and SLN metastases: HLA-A,B,C level was significantly lower in SLN LCs from thick lesions patients compared with those from thin/intermediate lesions (p < 0.05); β2-microglobulin level was significantly higher in positive SLN LCs compared to negative ones (p < 0.05). Functionally, SLN LCs did not phagocytose exogenous antigens. These findings extend LCs knowledge indicating that they are not fully impaired by melanoma, contributing to design new LCs-based therapeutic approaches.
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Authors
Maria Raffaella Romoli, Paola Di Gennaro, Gianni Gerlini, Serena Sestini, Paola Brandani, Soldano Ferrone, Lorenzo Borgognoni,