Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8467251 | Developmental Biology | 2018 | 33 Pages |
Abstract
During vertebrate development, progenitor cells give rise to tissues and organs through a complex choreography that commences at gastrulation. A hallmark event of gastrulation is the formation of the primitive streak, a linear assembly of cells along the anterior-posterior (AP) axis of the developing organism. To examine the primitive streak at a single-cell resolution, we measured the transcriptomes of individual chick cells from the streak or the surrounding tissue (the rest of the area pellucida) in Hamburger-Hamilton stage 4 embryos. The single-cell transcriptomes were then ordered by the statistical method Wave-Crest to deduce both the relative position along the AP axis and the prospective lineage of single cells. The ordered transcriptomes reveal intricate patterns of gene expression along the primitive streak.
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Authors
Katie L. Vermillion, Rhonda Bacher, Alex P. Tannenbaum, Scott Swanson, Peng Jiang, Li-Fang Chu, Ron Stewart, James A. Thomson, David T. Vereide,