Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8468102 | Developmental Biology | 2011 | 11 Pages |
Abstract
⺠Previous studies have shown that antisense morpholino oligonucleotides (MOs) can have off-target effects mediated by activation of Tp53 and associated apoptosis. ⺠We show that in the zebrafish hindbrain, MOs can also non-specifically induce ectopic boundary marker gene expression. ⺠The induction of ectopic gene expression by MOs is due to a normal non-apoptotic role of the Bcl-caspase pathway that is required for hindbrain boundary gene expression. ⺠These findings reveal that a previous study suggesting that Wnt1 promotes neurogenesis and restricts hindbrain boundary marker expression is incorrect. ⺠The finding that MOs can induce non-apoptotic roles of the pro-apoptotic pathway further emphasizes the need for precautions in their use and interpretation of results.
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Authors
Sebastian S. Gerety, David G. Wilkinson,