Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8470702 | Fungal Genetics and Biology | 2015 | 10 Pages |
Abstract
Candida albicans is a common pathogenic fungus and has aroused widespread attention recently. Actin cytoskeleton, an important player in polarized growth, protein secretion and organization of cell shape, displays irreplaceable role in hyphal development and cell integrity. In this study, we demonstrated a homologue of Saccharomyces cerevisiae Sac1, in C. albicans. It is a potential PIP phosphatase with Sac domain which is related to actin organization, hyphal development, biofilm formation and cell wall integrity. Deletion of SAC1 did not lead to insitiol-auxotroph phenotype in C. albicans, but this gene rescued the growth defect of S. cerevisiae sac1Î in the insitiol-free medium. Hyphal induction further revealed the deficiency of sac1Î/Î in hyphal development and biofilm formation. Fluorescence observation and real time PCR (RT-PCR) analysis suggested both actin and the hyphal cell wall protein Hwp1 were overexpressed and mislocated in this mutant. Furthermore, cell wall integrity (CWI) was largely affected by deletion of SAC1, due to the hypersensitivity to cell wall stress, changed content and distribution of chitin in the mutant. As a result, the virulence of sac1Î/Î was seriously attenuated. Taken together, this study provides evidence that Sac1, as a potential PIP phosphatase, is essential for actin organization, hyphal development, CWI and pathogenicity in C. albicans.
Keywords
FBSPhosphatidylinositol-4-phosphateActin organization5-FOACWICFWGPicAMPPI(3)PPI(4)PCyclic adenosine monophosphate5-fluoroorotic acidHyphal developmentfetal bovine serumPhosphatasephosphatidylinositolphosphatidylinositol-3-phosphatesynthetic completeCandida albicanscalcofluor WHITEcell wall integrity
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Authors
Bing Zhang, Qilin Yu, Chang Jia, Yuzhou Wang, Chenpeng Xiao, Yijie Dong, Ning Xu, Lei Wang, Mingchun Li,