Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8471056 | Gene Expression Patterns | 2018 | 6 Pages |
Abstract
In this paper, two cysteine proteinases were cloned from Jatropha curcas seeds. The full length cDNAs obtained from cloning of Jc-CysEP1 and Jc-CysEP2 genes were 1.516bp and 1500â¯pb, respectively. The Jc-CysEP1 contained a 1083bp open reading frame (ORF) coding for 360 amino acids. The JcCysEP1 protein sequence had an estimated native molecular weight of 36.89â¯kDa, with a predicted isoelectric point of 4.55. The average lengths of JcCysEP1 5â² UTR and 3â² UTR were 269 bp and 167bp, respectively. The Jc-CysEP2 contained a 1077â¯pb open reading frame (ORF) that encoded 358 amino acids. We also identified UTRs with lengths of 229â¯pb (5â²UTR) and 194â¯pb (3â²UTR). The Jc-CysEP2 sequence had a native molecular weight of 39.94â¯kDa, with a predicted isoelectric point of 6.19. Real-time PCR analyses of developing seeds (stages I-VII) showed that most cysteine proteinase genes were expressed at stage IV (middle stage) revealing peculiar spatio-temporal differences. JcCysEP2 was the cysteine proteinase gene with the highest expression in inner integument tissue, while JcCysEP1 was expressed in lower levels. Our results suggest that JcCysEP2 could be the major cysteine proteinase gene involved in PCD events in inner integument tissue, playing a critical role in PCD events during seed development, while Jc-CyEP1 and JcCysEP2 genes act cooperatively in stages IV-VII. JcCysEP2 is important to complete their participation in PCD until development of seeds.
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Authors
Antônio José Rocha, Simone Pohl, Cristiane Sá Roriz Fonteles,