Production d'un réactif monoclonal anti-B humain pour la détection des groupes sanguins ABO

The major part of monoclonal antibodies has a murine origin. They are obtained by the murine hybridoma technology. However, in the absence of an animalery, the transformation technology of human B-lymphocytes by Epstein-Barr virus (EBV) can be an alternative. The aim of this study is to produce a human monoclonal antibodies use in the determination of ABO blood grouping. A sampling of blood was deducted from a woman whose her blood type was O rhesus negative. EBV transformed lymphocytes obtained by density-gradient separation. Lymphoblastoïde cells were consolidated by fusion with P3X63Ag-8 murine myeloma cells according to the hybridoma technology, then cloned by limiting dilutions. A H04 human monoclonal antibody anti-B was selected. It recognizes the B, AB, B3 and Bx erythrocytes. The comparison of its reactivity with other anti-B commercial antibodies didn't show any discordance. The H04 human monoclonal antibody can be used like a reagent for the determination of ABO blood types and the study of antigen B epitopes.