Development of a commercial LEDs-based fluorescence polarization microplate reader for molecular biology measurements: System performance evaluation using calibration standards

In this work, the design of a commercial LEDs-based fluorescence polarization microplate reader which can be used for molecular bioanalytical measurements is described. The obtained validation measurements for the fluorescence polarization microplate reader indicate that the detection limit is 5 mP at 10 nM fluorescein (1 s integration time) measured with a black 96-well plate and the instrument dynamic range is >5 decades. Furthermore, the microplate reader fluorescence measurement mode can detect 1.5 fmol/200 μL fluorescein at 1 s integration time setting using a 96-well black microplate.